JASCO Time-Resolved Fluorescence Measurements with the FP-8050 Series (https://jascoinc.com/products/spectroscopy/fluorometer/details/)

The FP-8050 Series spectrofluorometers can measure five different types of spectra: emission, excitation, synchronous, single-beam emission and single-beam excitation in both fluorescence and phosphorescence* modes.

*Excludes FP-8250

Time Course Measurement
The Time Course Measurement program is intended for measuring temporal changes in fluorescence intensity at a fixed wavelength. Up to 100,000 hours (FP-8350/8550/8650) and 1667 hours (FP-8250) of continuous measurements can be performed using a 60 minute and 60 second interval, respectively.

Quantitative Analysis
In the Quantitative Analysis software, optimal parameters from two photometric modes, emission and excitation, and three quantitation modes, no base (1 wavelength), one-point base (2 wavelengths), and  two-point base (3 wavelengths) can be selected depending on the application. Other quantitative calibration curves methods such as log or spline functions are also available.

Fixed Wavelength Measurement
This program can be used to measure a sample’s fluorescence or phosphorescence intensity at fixed excitation and emission wavelengths for up to four wavelengths.

Phosphorescence Lifetime Measurement
Measures changes in the phosphorescence of a sample briefly irradiated by the excitation source.

*Excludes FP-8250

Interval Scan Measurement
Measures up to three spectra (fluorescence, excitation, and synchronous) and displays the results as either 2D or 3D spectra, as well as contour or color-coded plots.

Spectral Correction
Allows users to easily compare measured spectral data from several instruments as well as determine the quantum yield efficiency. Corrected spectra can be obtained immediately after the measurement is completed. The FP-8250/8350 require optional jigs for spectral correction. A Rhodamine B ethylene glycol solution is also included as a standard and additional sources for correction can be obtained separately.

Absorbance Spectra Measurement
Obtains the transmittance, absorbance, or reflectance spectrum by measuring the synchronous spectrum of a sample. The optional FUV-830 Absorbance measurement cell block is required for absorbance and transmittance measurements while reflectance measurements require an integrating sphere.

Relative Quantum Yield
All models in the FP-8000 Series include a relative quantum yield calculation program as standard.

3D Measurements
Allows for the simultaneous display of several different sets of data, including 2D, 3D, and synchronous spectra. The 3D plots can be viewed in Contour, Color 3D, and Color-mapping.

Validation
The validation program includes instrument test procedures in compliance with JIS (K 0120 2005) and JAIMAS (0004-2005). This program provides six performance tests including wavelength accuracy, wavelength repeatability, resolution, stray light, sensitivity, and photometric stability. The test results and procedures can be saved and/or printed.

JASCO : Time resolved Measurements with FTIR (https://www.jasco-global.com/solutions/time-resolution-measurements-with-ftir/)

Introduction

Q. We want to conduct time-resolved measurements with FTIR. What is the level of time resolution available?
A. FT-IR spectroscopy has 3 methods of time-resolved measurements: interval, rapid-scan, and step-scan measurements. The minimum time resolution for these methods is 1 second, 50 milliseconds and 5 microseconds (optional: 10 nanoseconds), respectively.

Each method’s feature and an example measurement is provided here. Each method has a different type of scanning method of the moving mirror within the interferometer. The “Interval measurement” is performed with regular scanning at a controlled interval, the “rapid-scan measurement” is obtained with rapid scanning of the sample during a minimal time variation. For “step-scan measurements”, the moving mirror makes a stop at every data-sampling point and mirror perturbation is performed at the various data-sampling points.

Interval Measurement*1

Time Resolution: 1 second
Applications: structural transition of a sample, monitoring of gas concentrations
Measurement example: A transition of the CO2 concentration in room air was measured (Figure 1). Interval measurement is effective for extended monitoring of a gas concentration.
*1JASCO ‘Interval Scan Measurement’ program can provide a maximum of 60,001 data points or a 24 day measurement maximum.

Figure 1. CO2 concentration transition (Interval measurement data, left: 3D spectrum, right: peak intensity transition of 2360 cm-1)

Rapid-scan measurement

Time Resolution: 50 milliseconds
Applications: photo-polymerization reaction, orientation relaxation of polymer film
Measurement example: The curing process of a UV cured resin was measured, which represents a photopolymerization reaction (Figure 2). The peak intensity of the C=C stretching peak at 1637 cm-1 (Figure 2) rapidly decreases responding to the UV irradiation, indicating the accurate observation of the initial reaction and the total reaction process.

Figure 2. Cure process of UV cured resin (Rapid-scan measurement data, left: 3D spectrum, right: peak intensity transition of 1637 cm-1)

Step-scan measurement*2

Time Resolution: 5 microseconds *3
Applications: relaxation process of a liquid crystal orientation
Measurement example: The relaxation process of a liquid crystal orientation stimulated by an electric field was measured (Figure 3). Specifically, the peak intensity at 2925 cm-1, a C-H stretching vibration, is observed to respond to the applied voltage. The peak change also decreases in two phases, rapidly and then moderately, after the power is turned off. It is known that the liquid crystals near the electrode interface has a faster orientation relaxation than the bulk crystal and the difference between these sites is captured with this measurement.
*2 limited to materials with repetitive, reproducible reactions;
*3 a 10 nanosecond response system is available as an option

Figure 3. Relaxation process of a liquid crystal (Step-scan measurement data, left: 3D spectrum, right: peak intensity transition of 2925 cm-1)